Journal: International Journal of Molecular Sciences
Article Title: Metabolic and Antioxidant Modulation by Artemisia indica Willd. Aqueous Extract in Glucose and Cholesterol Dysregulation
doi: 10.3390/ijms27010297
Figure Lengend Snippet: AAE inhibited polyol pathway activity and glycogen accumulation in HFD/STZ mice. The levels of aldose reductase ( a ), fructose ( b ), methylglyoxal ( c ), and carboxymethyl-lysine ( d ) were detected by a commercially available ELISA kit. ( e ) Pathological changes were evaluated by periodic acid–Schiff (PAS) staining, showing glycogen accumulation and abnormal polysaccharides (400× magnification). ( f ) PAS-positive areas in kidney tissues were quantified. Data were collected per group and are presented as the mean ± SD ( n = 5), derived from five independent biological replicates. # p < 0.05, ## p < 0.01 versus the control group; * p < 0.05, ** p < 0.01 versus the model group. AAE: Artemisia indica Willd. aqueous extract; HFD: high-fat diet; PAS: periodic acid–Schiff; STZ: streptozotocin.
Article Snippet: A normalized analysis was conducted by measuring urinary albumin (Mouse Albumin AssayMax ELISA Kit, Assaypro, Saint Charles, MO, USA, Cat# EMA3201-1) and urinary creatinine (Cayman Creatinine ELISA Kit, Item No. 502330, Michigan, USA), and subsequently, the urinary albumin-to-creatinine ratio (UACR) was calculated [ ].
Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Staining, Derivative Assay, Control